Pattern of ROS generation and interconversion on wet stigmas in basal and divergent angiosperms

The active participation of ROS in the reproductive physiology flowering plants is well-documented model species, mainly with dry stigmas. Here we studied release into stigma exudate—a fluid involved pollen acceptance on a wet stigma. study from different systematic groups: Paeonia suffruticosa, Nicotiana tabacum, Lilium hybr., Aristolochia manshuriensis and Berberis vulgaris; most cases, exudate was collected intact flowers whole plant to minimize effect experimental intervention sensitive redox balance system. dynamics total oxidizing capacity assessed by EPR spectroscopy staining. level hydrogen peroxide activity superoxide dismutase, which catalyzes H2O2 formation, also evaluated. In all studied, increased maturation, however, concentration decreased representatives eudicots, except for peony. lily it increased, while birthwort remained low, apparently due absence SOD activity. divergent enzyme stigmas two isoenzymes. During fertile period, higher than at an earlier stage. Based obtained data, speculate primitive progressive patterns generation transformation its possible evolution.